Chai Biotechnologies Launches Kickstarter Campaign For An Open-Source Real-Time PCR Thermocycler

Open QPCR 3

On Tuesday (November 4th), Chai Biotechnologies launched a Kickstarter crowdfunding campaign for its Open qPCR, the dubbed   world’s “first open source Real-Time PCR Thermocycler.” The instrument, which is capable of genetic genotyping, pathogen presence/absence detection, and relative DNA quantification.

Open QPCR 2Real-Time PCR is used in diagnostic applications ranging from food safety to viral diagnostics, as well as numerous research applications.  Founder and Chief Executive of Chai, Josh Perfetto stated, “With Real-Time PCR thermocyclers typically costing $20,000 and up, this technology was simply unaffordable to lesser funded university labs, institutes in developing countries dealing with emerging epidemics, and the growing DIYbio/maker movement. With Open qPCR, we’ve lowered the cost of the Real-Time PCR reaction by an order of magnitude.”

Perfetto was earlier the co-creator of the OpenPCR project, which brought the world first open source endpoint PCR thermocycler to market in 2011. With its 16 well sample capacity (100 or 200 uL tubes), Open qPCR is primarily focused on diagnostic applications.

Features of the new qPCR device include:

  • Open QPCR 1Visual, web-based interface for protocol editing, plate layout, and results interpretation
  • Integrated touch screen supports field use
  • Ethernet, wifi, and USB connectivity
  • Open API supports automation applications
  • Low power mode for isothermal amplification applications
  • Complete hardware design and software to be released as open source when machine ships

The campaign runs until December 4th. The campaign is also raising funds to donate instruments to Ebola clinics in Western Africa. Open qPCR is currently available for $1499 as part of the campaign, and is expected to ship in March 2015.

Chai Biotechnologies is a biotech startup in Santa Clara, California focused on making the core molecular biological technologies accessible to all, while developing powerful tools for the next generation of synthetic biologists. Chai was founded to develop the Open qPCR  thermocycler announced in this release, and is also the manufacturer of OpenPCR endpoint thermocyclers.

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